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Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals) [Hardcover]

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Item description for Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals) by Thomas Köhler...

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.



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Item Specifications...


Pages   166
Est. Packaging Dimensions:   Length: 9.25" Width: 6.73" Height: 0.66"
Weight:   0.73 lbs.
Binding  Hardcover
Release Date   Nov 27, 1995
Publisher   Springer
ISBN  3540591923  
ISBN13  9783540591924  


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