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Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution [Hardcover]

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Item description for Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution by Susanne Brakmann...

Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed.

From library generation by random or directed mutagenesis to screening and selection techniques---the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems.

Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution.

As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms.

In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.

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Item Specifications...

Pages   227
Est. Packaging Dimensions:   Length: 9.6" Width: 6.7" Height: 0.5"
Weight:   1.15 lbs.
Binding  Hardcover
Release Date   Aug 20, 2004
Publisher   Wiley-VCH
ISBN  3527307990  
ISBN13  9783527307999  

Availability  0 units.

More About Susanne Brakmann

Register your artisan biography and upload your photo! Susanne Brakmann is head of the junior research group "Applied Molecular Evolution" at the University of Leipzig (Germany) and a Member of the Biotechnological-Biomedical Center of Leipzig. She studied Chemistry at the Technical University of Braunschweig where she received her diploma in 1988, moving afterwards to the University of Karlsruhe to work on her thesis under the supervision of Reinhold Tacke (Ph. D. 1991). She was postdoctoral fellow at the Max-Planck-Institute for Biophysical Chemistry in Gottingen where she worked with Manfred Eigen before she moved to Leipzig in 2001. She is interested in directed evolution as a tool for understanding and optimizing enzyme functions, focusing on nucleic acid polymerases and their biotechnological applications.

Andreas Schwienhorst is a group leader at the Institute of Microbiology and Genetics at the University of Gottingen (Germany). He was born in Warendorf and did his studies of Biochemistry and Prehistory in Munster and Gottingen. His thesis was carried out at the Max-Planck-Institute for Biophysical Chemistry under the supervision of Manfred Eigen. He was a visiting scientist at the Salk Institute, La Jolla and the Los Alamos National Laboratory (USA), then took a position as a group leader at the Institute for Molecular Biotechnology before moving to the University of Gottingen in 1997. In 1998 he received the Biofuture Award. Currently, he is interested in the discovery of novel targets for drug intervention as well as in methods of molecular evolution and their applications in drug discovery and catalysis.

Susanne Brakmann has an academic affiliation as follows - University of Leipzig, Germany.

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Product Categories

1Books > Foreign Language Books > German > All German Books
2Books > Foreign Language Books > German > Nonfiction
3Books > Foreign Language Books > German > Professional & Technical
4Books > Subjects > Medicine > Special Topics > Biotechnology
5Books > Subjects > Medicine > Specialties > Pathology > Clinical Chemistry
6Books > Subjects > Professional & Technical > Engineering > Bioengineering > Biotechnology
7Books > Subjects > Professional & Technical > Engineering > Chemical > General
8Books > Subjects > Professional & Technical > Medical > Basic Sciences > Biotechnology
9Books > Subjects > Professional & Technical > Medical > Medicine > Internal Medicine > Pathology > Clinical Chemistry
10Books > Subjects > Professional & Technical > Professional Science > Chemistry > Organic
11Books > Subjects > Science > Evolution > General
12Books > Subjects > Science > General

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