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Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis [Hardcover]

By Susanne Brakmann (Editor) & Kai Johnsson (Editor)
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Item description for Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis by Susanne Brakmann & Kai Johnsson...

Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity.
The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!



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Item Specifications...


Pages   368
Est. Packaging Dimensions:   Length: 0.8" Width: 6.75" Height: 9.5"
Weight:   1.85 lbs.
Binding  Hardcover
Release Date   May 6, 2002
Publisher   Wiley-VCH
ISBN  3527304231  
ISBN13  9783527304233  


Availability  0 units.


More About Susanne Brakmann & Kai Johnsson


Register your artisan biography and upload your photo! Susanne Brakmann is head of the junior research group "Applied Molecular Evolution" at the University of Leipzig (Germany) and a Member of the Biotechnological-Biomedical Center of Leipzig. She studied Chemistry at the Technical University of Braunschweig where she received her diploma in 1988, moving afterwards to the University of Karlsruhe to work on her thesis under the supervision of Reinhold Tacke (Ph. D. 1991). She was postdoctoral fellow at the Max-Planck-Institute for Biophysical Chemistry in Gottingen where she worked with Manfred Eigen before she moved to Leipzig in 2001. She is interested in directed evolution as a tool for understanding and optimizing enzyme functions, focusing on nucleic acid polymerases and their biotechnological applications.

Andreas Schwienhorst is a group leader at the Institute of Microbiology and Genetics at the University of Gottingen (Germany). He was born in Warendorf and did his studies of Biochemistry and Prehistory in Munster and Gottingen. His thesis was carried out at the Max-Planck-Institute for Biophysical Chemistry under the supervision of Manfred Eigen. He was a visiting scientist at the Salk Institute, La Jolla and the Los Alamos National Laboratory (USA), then took a position as a group leader at the Institute for Molecular Biotechnology before moving to the University of Gottingen in 1997. In 1998 he received the Biofuture Award. Currently, he is interested in the discovery of novel targets for drug intervention as well as in methods of molecular evolution and their applications in drug discovery and catalysis.

Susanne Brakmann has an academic affiliation as follows - University of Leipzig, Germany.

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1Books > Subjects > Medicine > Basic Science > Biochemistry
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