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Proteome Research: Mass Spectrometry (Principles and Practice) [Paperback]

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Item description for Proteome Research: Mass Spectrometry (Principles and Practice) by Peter James...

Recent advances in large-scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Two-dimensional gel electrophoresis can be used to create cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale idenfification of these proteomes and their modifications. An understanding of these methods is critical for scientists in the "Post-Genome" era.

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Item Specifications...

Pages   274
Est. Packaging Dimensions:   Length: 9.12" Width: 6.11" Height: 0.57"
Weight:   1.09 lbs.
Binding  Softcover
Release Date   Dec 12, 2000
Publisher   Springer
ISBN  3540672567  
ISBN13  9783540672562  

Availability  144 units.
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More About Peter James

Register your artisan biography and upload your photo! Peter James is a professional writer on ancient history and archeology. He studied at Birmingham and London universities and describes himself as a "generalist" in the study of the ancient Near East and Mediterranean. He has published numerous articles on ancient technology, chronology, and the history of science and is the principal author of the highly controversial Centuries of Darkness and a forthcoming book on Atlantis, The Sunken Kingdom.
Dr. Nick Thorpe, an archeologist in prehistory, studied at Reading and London universities and is now lecturer in archeology at King Alfred's College, Winchester. He directs research projects in Britain and Denmark. He has contributed articles on agriculture and chronology, metalworking, astronomy, and prehistoric society to numerous books and journals and is a coauthor of Centuries of Darkness.

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1Books > Bargain Books > Children > Science, Nature & How It Works
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Reviews - What do customers think about Proteome Research: Mass Spectrometry (Principles and Practice)?

A protein mass spectrometry book in disguise  Jul 3, 2001
Although the title of this book adopts the term "proteome" (no doubt to aid sales) it describes essentially mass spectrometry of peptides and proteins. After an introductory chapter by the editor, the next chapters are devoted to specific mass spectrometers, namely electrospray ionisation on triple quadrupoles and matrix-assisted laser desorption ionisation on time-of-flight instruments. Three chapters describe MS approaches to identify proteins including mass fingerprinting and sequencing with the aid of automated tandem MS/MS spectra interpretation. The analysis of post-translational modifications, in the form of phosphorylation and glycosylation, is described followed by a comprehensive chapter of protein databases. The book ends with chapter discussing instrument configurations and somewhat of a focus on FT-ICR's. What the book lacks is an inclusion of applications in proteome analysis by mass spectrometry. This is probably because there is very little published data on the subject, while the "proposed" research has received considerable mileage. Another curious feature of this text is the selection of authors. Of particular note is the editor who tackles the chapters on protein mass fingerprinting and interpreting tandem mass spectrometry despite a lack of publications in both areas. Little wonder then section 8.2.4 spends some time discussing charge-localisation and its influence on peptide fragmentation for low energy MS/MS spectra and not high-energy collision spectra where this phenomenon is far more evident. In short, a possibly useful book if you have the dollars to spare. If not, head to your book shelf or library for one on protein mass spectrometry. There's not much new here.

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