Item description for Flow Cytometry and Cell Sorting (Springer Lab Manual) by Andreas Radbruch...
Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced. In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies.
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Est. Packaging Dimensions: Length: 9.59" Width: 8.47" Height: 1.09" Weight: 2 lbs.
Binding Spiral Bound
Release Date Dec 10, 1999
ISBN 3540656308 ISBN13 9783540656302
Availability 142 units. Availability accurate as of Oct 28, 2016 08:39.
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Reviews - What do customers think about Flow Cytometry and Cell Sorting (Springer Lab Manual)?
A book for the beginner and a review for the experienced Jun 16, 2000
This is the first book I purchased for flow cytometry after checking out 3 unsatisfactory books on the same subject from the library. I am a clinician by training and so I needed a book that explains the basic background of the method, PLUS detailed procedures to follow, and explanation on how to alter those procedures to suit one's experiment. This book is written and presented in a way that makes it easy to understand even if one has no prior trianing in flow-cytometry. It does not have so much technical jargon that a newbie would not be able to understand. Everything is clear and well-explained. I have "debugged" some of my current procedures using this book. Chapter 3 (Immunofluroescence: Basic Considerations) is a must-read: the validity of one's results seem to largely depend on factors discussed in this chapter. Included also is a list of internet resouce for cytometry, although some of websites have been outdated. Overall a very nice reference, but as in all lab procedures-related books, one book never covers everything you need to know, so I use this book by Radbruch in conjuction with others.